The detection of circulating breast cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
The detection of circulating breast cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction.
Some circulating cancer cells in the blood play a central role in the metastatic process and may have a major influence on patient progress. Their numbers can be very small and techniques for their detection need to be both sensitive and specific. Polymerase chain reaction (PCR) has been successfully used to detect small numbers of tumor cells in cancer. We used a reverse transcriptase-polymera...
متن کاملDetection of circulating breast cancer cells in peripheral blood by a two-marker reverse transcriptase-polymerase chain reaction assay.
The aim of this study was to use a two-marker assay for the detection of breast cancer cells circulating in patients' blood. We have applied a PCR-based methodology to follow up the possibility of the development of metastatic disease in stage I and II patients who had undergone curative surgery. Since the number of circulating cancer cells in peripheral blood is very low, the technique for the...
متن کاملSensitive detection of circulating breast cancer cells by reverse-transcriptase polymerase chain reaction of maspin gene.
BACKGROUND Maspin, a recently identified protein related to the family of serpins, is believed to play a role in human breast cancer. In an effort to improve the present methods of detection, we have developed a reverse-transcriptase polymerase chain reaction (RT-PCR) assay for maspin transcript to identify small numbers of mammary carcinoma cells in the peripheral blood and bone marrow of pati...
متن کاملSensitive detection of rare circulating neuroblastoma cells by the reverse transcriptase-polymerase chain reaction.
The presence of circulating tumor cells in patients with localized or disseminated neuroblastoma may be a significant prognostic factor at diagnosis and may antedate the detection of relapse by other diagnostic studies. We report the development of a highly sensitive detection assay for circulating neuroblasts based on the reverse transcriptase-polymerase chain reaction (RT-PCR), using the neur...
متن کاملDetection of micrometastatic prostate cancer cells in lymph nodes by reverse transcriptase-polymerase chain reaction.
We have developed a highly sensitive method for detecting prostate cancer cells using reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific for prostate-specific antigen gene. Forty-four lymph nodes obtained from 22 patients with prostate cancers were analyzed by RT-PCR to detect metastatic prostate cancer cells. RT-PCR could detect prostate-specific antigen mRNA in fiv...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Korean Medical Science
سال: 2000
ISSN: 1011-8934,1598-6357
DOI: 10.3346/jkms.2000.15.2.194